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. 2017 Sep 19;16(5):7205–7212. doi: 10.3892/mmr.2017.7524

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Copyright: © Pan et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 3. — Analysis of the effects of treatment with 4-PBA in HAECs exposed to d-flow. (A) Results of western blotting of GRP78, CHOP, caspase 12 and GAPDH in HAECs treated with 4-PBA and/or d-flow. The relative expression of GRP78 (white columns) and CHOP (black columns) in HAECs treated with 4-PBA and/or d-flow was normalized to GAPDH. (B) The left panel exhibits the captured images from the terminal deoxynucleotidyl transferase dUTP nick end labeling assay of HAECs treated with 4-PBA and/or d-flow. The detected apoptotic percentages of HAECs treated with 4-PBA and/or d-flow were quantified. Magnification, ×200. *P<0.05 vs. Ctrl; ^#P<0.05 vs. Ctrl+4-PBA; ^&P<0.05 vs. d-flow. HAECs, human aortic endothelial cells; d-flow, disturbed blood flow; GRP78, 78 kDa glucose-regulated protein; CHOP, DNA damage-inducible transcript 3 protein; 4-PBA, 4-phenylbutyric acid; Ctrl, control.