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. 2017 Sep 20;16(5):7305–7314. doi: 10.3892/mmr.2017.7547

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Copyright: © Zhou et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 2. — Microphotographs indicating GFAP staining (red) and lentivirus infection (green) in astrocytes. Astrocytes were successfully infected with (A) Plenti-GFP lentivirus as an overexpression control, (B) Plenti-TREK-1-GFP lentivirus for TREK-1 overexpression, (C) shRNA-sham-GFP lentivirus as a knockdown control and (D) shRNA-TREK-1-GFP lentivirus for knockdown of TREK-1. Scale bar, 200 µm. GFAP, glial fibrillary acidic protein; GFP, green fluorescent protein; TREK-1, TWIK-related K⁺ channel; shRNA, short hairpin RNA.