Table II.
Primers used for mutation screening of the FBN1 gene.
| Primer name | Primer sequence (5′-3′) | Product size (bp) | Annealing temperature (°C) |
|---|---|---|---|
| FBN11&2F | TCGGGGATTTGTCTCTGTGT | 434 | 59 |
| FBN11&2R | GCCCGTTGTTCTGGATCTTG | ||
| FBN13F | ACCAACCCAGCATTGAGTCT | 308 | 60 |
| FBN13R | TTCTAAGGCTCCCCATGCAA | ||
| FBN14F | TTGTGAGGGACCTGAGAACC | 296 | 59 |
| FBN14R | TTGCAGGAAAGAGGAAAGCC | ||
| FBN15F | CAACTCCTGTGAGCTGTTGC | 278 | 60 |
| FBN15R | AAACATGCTGTGTCCCAGGT | ||
| FBN16F | GTCCTTCCAGAGGACCACAA | 228 | 60 |
| FBN16R | CAGCTTTAGGTACCAGCATGTC | ||
| FBN17F | GCATGATGGTTCCTGCTTTT | 380 | 60 |
| FBN17R | GCAGTCAGCGAAATTGTGAA | ||
| FBN18F | TTCCAAATATTGTGATGGACAAA | 448 | 60 |
| FBN18R | ACAGGGTTTTTCTGGTCCAA | ||
| FBN19F | GCTGTTTCCAGGGACATGAT | 441 | 60 |
| FBN19R | TTTATGGGAGGCAAAACGTC | ||
| FBN110F | AGCCCCAGTGTGAAGTATGG | 396 | 60 |
| FBN110R | TTCCCTGGACGTCATCTCTT | ||
| FBN111F | TGACTTCTGTGGGCCTATGA | 300 | 59 |
| FBN111R | TTAACTTGAACAATGCAAGAAAAA | ||
| FBN112F | TTGTCACCAGACGACCTTTG | 383 | 60 |
| FBN112R | CCACCAAGTTTGGGGTAAGTT | ||
| FBN113F | AAAAGGAACCCAGAAAGTCTTAGAA | 295 | 60 |
| FBN113R | CTTCCGGCATGGGTTATTTA | ||
| FBN114F | GGAGGGAGGGGGAAATAAA | 244 | 60 |
| FBN114R | ACTGCAATGGAAGGAGAGGA | ||
| FBN115F | GATCTTATTTGGATGAAAGTTAGCC | 400 | 59 |
| FBN115R | AGTCAGGTTTCCCAAACCAA | ||
| FBN116F | TTCCCCATTTTCAAGGGTTA | 294 | 61 |
| FBN116R | CGTTTGTTACCATTGGGCTTT | ||
| FBN117F | GGGGGTTCTCATCTGTTTGA | 242 | 60 |
| FBN117R | CAGTACGAGGGCATCTCCAT | ||
| FBN118F | ACCAAGGGCAGGATCTACCT | 188 | 60 |
| FBN118R | ACCCACAAGAAAGCCTGATG | ||
| FBN119F | CCTGTAGCTCCTAAGGTCATTACA | 300 | 60 |
| FBN119R | CTCCCAGCAATGAAAGAAGG | ||
| FBN120F | CAAAGTTTGGGCCCTTTTTA | 226 | 59 |
| FBN120R | TGGCATTCCAAAAGATAGCA | ||
| FBN121F | GGCCCAAGACTAGATTTTAGCA | 243 | 60 |
| FBN121R | TTTTGCAGGAAAAGCTGACA | ||
| FBN122F | AATGTCAGCTTTTCCTGCAA | 368 | 59 |
| FBN122R | TGAAATACTAGGCTTCCCCTTT | ||
| FBN123F | TGTCAGAACTGCAAAGTCTGG | 204 | 60 |
| FBN123R | GACAGCTTTATCCAGTCCGAGT | ||
| FBN124F | TGCTATTCAGGCACCCTAGA | 400 | 59 |
| FBN124R | TGGAGTGTGTGTCTGTACCTGA | ||
| FBN125F | AACAGAGTGTTGGCAGTTTGG | 373 | 60 |
| FBN125R | CTGAGATCATGAAAATGCATCC | ||
| FBN126&27F | GACCTCCTGACTGCTTGCTC | 494 | 60 |
| FBN126&27R | CAAAGCTTCATGGAATCCTTCT | ||
| FBN128&29F | GAGTGCTTGGTCTGGTGGAG | 564 | 61 |
| FBN128&29R | AGCGATGAAAACAAAACTCAGA | ||
| FBN130F | GGGACAGACATCCAAACCAT | 249 | 62 |
| FBN130R | CAAAGCCTGGGCCCTAAAC | ||
| FBN131F | CTCACTGAACAGTGGAACCAA | 280 | 59 |
| FBN131R | GCTCTCTTTGGAATGCTGGT | 280 | 59 |
| FBN132F | GAATCTTTCTATCACTGACCCAAAC | ||
| FBN132R | TCGAGGGGAAAGTACTCAATG | 325 | 59 |
| FBN133&34F | CATTTGTGCTGAGCCTTTTTC | 495 | 60 |
| FBN133&34R | GAATGCCTGGCTTCTCTGAC | ||
| FBN135F | TGCTGCACTGGAAAGTTGAT | 231 | 60 |
| FBN135R | AGTGGCTTCCCCATCAGTTA | ||
| FBN136F | TGCCCAGATTGGTGTTAGAT | 400 | 59 |
| FBN136R | CAGGTCTGAGAAAAGGTATCTGTG | ||
| FBN137&38F | AGATTGGGCCCTGTTCTTTT | 819 | 60 |
| FBN137&38R | TTGGGAATAAGGTCCCCTCT | ||
| FBN139&40F | TCAGACGGGCAGAGTAACAA | 496 | 59 |
| FBN139&40R | CCATATTCTGGTTTTGCAGGT | ||
| FBN141F | AGGCCATTCCAAAATGTGAA | 249 | 60 |
| FBN141R | TTGTGAGCTCTCTTCCTCTTTGT | ||
| FBN142F | ATTTCCCACATGGCATCAC | 300 | 60 |
| FBN142R | TGCTTCCTTCGCTAAGACTGA | ||
| FBN143F | CTATCCTCCCATCCCACCTT | 273 | 60 |
| FBN143R | CAGGGTGTTTGCACAGTTTG | ||
| FBN144F | CACAGGGATCATGTGCTGTC | 315 | 60 |
| FBN144R | TCCACACCATGCCCTTTACT | ||
| FBN145F | GGCTTTGTTGACTGGACACC | 218 | 62 |
| FBN145R | GTAGGCATGTCCAGCCTGTG | ||
| FBN146F | GAGCTAGGATTACTCCTGAGAATGA | 398 | 59 |
| FBN146R | TCATGTTCAGATTGCCAAAGA | ||
| FBN147F | GGCCTGGTGAACCCTAAAAT | 247 | 60 |
| FBN147R | TTCCTTTGCTGATGCACAAT | ||
| FBN148F | TGCTGGGATTATGACATCTTTG | 292 | 60 |
| FBN148R | TTTTCCTCCAGGTTTCCAGA | ||
| FBN149F | CCAGTGGGAACCTCTTCCTT | 205 | 60 |
| FBN149R | GACACCCGACACTCCTCATT | ||
| FBN150F | TGATGTCTCCATCGTGTTTTG | 208 | 61 |
| FBN150R | ATTGAAAGCCCAAAGCCTTC | ||
| FBN151F | GGAAAGCAACTGAAGGGTGT | 263 | 590 |
| FBN151R | GCCTACAGTCTTACTTACATCATGG | ||
| FBN152&53F | GGAGAAGCTTGTAATGAATTGCT | 594 | 60 |
| FBN152&53R | AACTTATTTCAGTGCCATCTTGG | ||
| FBN154F | TTTGGACACATTCCTGGTTTC | 207 | 60 |
| FBN154R | CAACCAATTGTTCCCAGGAT | ||
| FBN155F | CCTTTTGTTGCTGTCCATGAT | 249 | 60 |
| FBN155R | AGGGAAGCTTTGAGGGACAT | ||
| FBN156F | TCATACTCAACAGAGCAGAAGGA | 363 | 59 |
| FBN156R | CAAGAACTCAGAGCCCAGGT | ||
| FBN157F | AAGGAACAAAGGGAGGGAAG | 392 | 60 |
| FBN157R | CAGTCATTACGGCATCTCCA | ||
| FBN158F | CTGACATCCCCTTTGCCATA | 277 | 61 |
| FBN158R | TCCCTGCAAGTATTTTTGGAC | ||
| FBN159&60F | CACTGAAGTGACCCCCTACA | 600 | 60 |
| FBN159&60R | TGAGGGGCAATGGTCAAT | ||
| FBN161&62F | TGTTGGCTTGACTCAAATGC | 600 | 61 |
| FBN161&62R | CCTCCACAAGGATTCACCAG | ||
| FBN163F | TGGTGGCTCTGCTTCTTTTT | 178 | 60 |
| FBN163R | GCCATGCATCTTGAGAGTGA | ||
| FBN164F | AAGTGGCCAGATCCAATGTC | 334 | 60 |
| FBN164R | ACCATGACCAGGAAGAGCAC | ||
| FBN165F | CATCTATGCTCCCCTTCTGC | 243 | 60 |
| FBN165R | TTCCACCACAGGAGACATCA | ||
| FBN166F | GCAGCATAAGGCAGAAAATTG | 583 | 60 |
| FBN166R | TGATTCTGATTGGGGGAAAA |
FBN1, fibrillin-1; F, forward; R, reverse; bp, base pair.