Table I.
Gene | Primer sequences (5′→3′) |
---|---|
Human Bcl-2 | Positive sequence: GTGGCC |
TTCTTTGAGTTCGG | |
Reverse sequence: GGCCGT | |
ACAGTTCCACAAAG | |
Human Bax | Positive sequence: ATGAAG |
ACAGGGGCCCTTTT | |
Reverse sequence: GCAATC | |
ATCCTCTGCAGCTC | |
Human caspase-3 | Positive sequence: ACTGGA |
CTGTGGCATTGAGA | |
Reverse sequence: GCACAA | |
AGCGACTGGATGAA | |
Human GAPDH | Positive sequence: CCAGAA |
CATCATCCCTGCCT | |
Reverse sequence: CCTGCT | |
TCACCACCTTCTTG | |
Human UGCG siRNA | Positive sequence: CGCGAA |
UCCAUGACAAUAUTT | |
Reverse sequence: AUAUUG | |
UCAUGGAUUCGCGTT | |
Negative control siRNA | Positive sequence: GCGACG |
AUCUGCCUAAGAUdTdT | |
Reverse sequence: AUCUUA | |
GGCAGAUCGUCGUCGCdTdT | |
Human GCS | Positive sequence: TTCATG |
TGTCATTGCCTGGC | |
Reverse sequence: AGCGTA | |
ATCTGTAGCGACCA |
GCS, glucosylceramide synthase; UGCG, UDP-glucose ceramide glucosyltransferase.