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. 2017 Sep 21;16(5):7355–7360. doi: 10.3892/mmr.2017.7580

Table I.

Primer sequences for qPCR analysis.

Gene Primer sequences (5′→3′)
Human Bcl-2 Positive sequence: GTGGCC
TTCTTTGAGTTCGG
Reverse sequence: GGCCGT
ACAGTTCCACAAAG
Human Bax Positive sequence: ATGAAG
ACAGGGGCCCTTTT
Reverse sequence: GCAATC
ATCCTCTGCAGCTC
Human caspase-3 Positive sequence: ACTGGA
CTGTGGCATTGAGA
Reverse sequence: GCACAA
AGCGACTGGATGAA
Human GAPDH Positive sequence: CCAGAA
CATCATCCCTGCCT
Reverse sequence: CCTGCT
TCACCACCTTCTTG
Human UGCG siRNA Positive sequence: CGCGAA
UCCAUGACAAUAUTT
Reverse sequence: AUAUUG
UCAUGGAUUCGCGTT
Negative control siRNA Positive sequence: GCGACG
AUCUGCCUAAGAUdTdT
Reverse sequence: AUCUUA
GGCAGAUCGUCGUCGCdTdT
Human GCS Positive sequence: TTCATG
TGTCATTGCCTGGC
Reverse sequence: AGCGTA
ATCTGTAGCGACCA

GCS, glucosylceramide synthase; UGCG, UDP-glucose ceramide glucosyltransferase.