Figure 2.

ADMA suppresses Bcl2 and increased cleaved caspase-3 expression in HUVECs. (A-D) Cells were treated with the indicated concentration of ADMA (0–200 µM) for 24 h, or incubated with 100 µM ADMA for the indicated time periods. Bcl2 protein levels in cell extracts were analyzed by western blotting. Results are the mean ± SD (n=3). *P<0.05 compared with control. (E and F) Cells were treated with ADMA at the indicated dose (0–200 µM) and time period (0–48 h). Cell extracts were subjected to western blotting using antibodies against cleaved caspase-3.