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Time-dependent effects of rBmpA on the expression of NF-κB p65 and IκB-β mRNA at 12–24 h. BV2 microglia were stimulated by 20 mg/ml rBmpA for 48 h. The levels of (A) NF-κB p65 and (B) IκB-β mRNA were determined by reverse transcription-quantitative polymerase chain reaction. Each value represents the mean ± standard error of the mean. n=3. *P<0.05; **P<0.01. NF-κB, nuclear factor κ-B; rBmpA, recombinant basic membrane protein A; IκB-β. inhibitor of NF-κB; LPS, lipopolysaccharide.
