Figure 3.

Total protein quantification was used to analyze the protein expression of NF-κB p65 and IκB-β in BV2 cells. The cells were stimulated with 20 µg/ml rBmpA or 1 µg/ml LPS at different time points (12, 24 and 48 h). (A) The total protein was measured by SDS-PAGE using a Stain-Free Gel. The expression of (B) NF-κB p65 and (C) IκB-β, exhibited as the mean ± standard error of the mean. n=3. *P<0.05; **P<0.01. NF-κB, nuclear factor κ-B; rBmpA, recombinant basic membrane protein A; IκB-β inhibitor of NF-κB; LPS, lipopolysaccharide.