Figure 2.

Effects of CoCl₂ on proteins involved in epithelial-mesenchymal transition in MiaPaCa2 cells. (A) The morphology of cells was detected by hematoxylin and eosin staining and observed from five randomly selected microscopic visual fields (magnification, ×200) following treatment with or without CoCl₂ for 24 h. Red arrow represented cells with spindle shape. (B) The mRNA expression levels of E-cadherin, N-cadherin, Snail, HIF-1α, Notch1 in MiaPaCa2 cells treated with or without CoCl₂ for 24 h was detected using reverse transcription-quantitative polymerase chain reaction. (C) The protein content of E-cadherin, N-cadherin, Snail, HIF-1α, Notch1 in MiaPaCa2 cells treated with or without CoCl₂ for 24 h was detected by western blotting. GAPDH was included as mRNA-loading and protein-loading control. Data were representative of three independent experiments and expressed as the mean ± standard error of the mean. ***P<0.001 vs. control. N, neural; E, epithelial; HIF-1α, hypoxia inducible factor-1α; NC, negative control; CoCl₂, cobalt II chloride.