Table I.

The experimental design of CyDye labelling for each individual retinal paired sample (n=5).

Gel no.	Cy2 dye (internal control)	Cy3 dye	Cy5 dye
Gel 1	Pool of all 10 eyes	Treated 1	Control 1
	(5 µg from each sample)	(50 µg)	(50 µg)
Gel 2	Pool of all 10 eyes	Control 2	Treated 2
	(5 µg from each sample)	(50 µg)	(50 µg)
Gel 3	Pool of all 10 eyes	Treated 3	Control 3
	(5 µg from each sample)	(50 µg)	(50 µg)
Gel 4	Pool of all 10 eyes	Control 4	Treated 4
	(5 µg from each sample)	(50 µg)	(50 µg)
Gel 5	Pool of all 10 eyes	Treated 5	Control 5
	(5 µg from each sample)	(50 µg)	(50 µg)

A total of 50 µg treated and un-treated control eyes were labelled with different dyes (Cy3 or Cy5 randomly). A total of 150 µg labelled proteins (treated, control and pooled standard) were loaded on each gel (gel 1-gel 5) for 2D difference gel electrophoresis run.