Table III.

A list of differentially expressed retinal proteins in response to −4D lens-induced myopia for 8 days.

Spot no.	DeCyder no. in Fig. 1	Protein name	Paired average ratioa	DeCyder P.value for fold change	MOWSE scoreb	NCBI GI no.c	Mascot cal. pI.d	Mascot Mw (kDa)e	Speciesf	Biological process	Molecular function
1	859	β-actin	−1.31	0.00042	294	49868	5.78	39.4	Mus musculus	Cell motility	i) ATP binding; ii) as cytoskeleton protein
2	657	Enolase 1	−1.22	0.003	481	87196501	6.37	47.6	Bos taurus	i) Glycolysis; ii) plasminogen activation	Catalysis of 2.phosphoglycerate to phosphoenolpyruvate during glycolysis
3	1133	Cytosolic malate dehydrogenase	−1.27	0.0034	165	55595921	7.57	38.9	Pan troglodytes	Oocyte maturation and embryo development	Malate-aspartate shuttle in glycolysis
4	1655	Ras-related protein Rab-11B/YPT3	+1.24	0.00028	391	763130	5.87	24.7	Homo sapiens	i) Protein transport; ii) small GTPase mediated	i) GTP binding; ii) GTPase activity signal transduction
5	1599	Protein-L-isoa spartate(D-aspartate) O-methyl transferase	−1.24	0.018	166	2507187	6.7	24.8	Homo sapiens	i) Protein amino acid methylation; ii) protein repair	i) Identical protein binding; ii) protein-L-isoaspartate (D-aspartate) O-methyltransferase activity
6	1639	PKM2 protein	−1.21	0.0091	155	73587283	8.62	62.0	Bos taurus	Glycolysis	i) Magnesium ion binding; ii) potassium ion binding; iii) protein binding; iv) pyruvate kinase activity
7	1954	X-linked eukaryotic translation initiation factor 1A	−1.24	0.00011	102	4503499	5.07	16.5	Homo sapiens	Translational initiation	Initiating translation activity
8	1947	pkm1 protein	+1.52	8.2e-005	256	74267860	6.71	18.6	Bos taurus	Protein amino acid dephosphorylation	i) Acid phosphatase activity; ii) non-membrane spanning protein tyrosine phosphatase activity

Proteins were identified by mass spectrometry with documented functions according to their GeneOntology.

^aPaired average ratio denotes fold difference between myopic and control retina. - and + indicate downregulation and upregulation in the myopic retina, respectively.

^bScore for the protein with significant match calculated by MOWSE scoring algorithm in the Mascot system.

^cA GI number is assigned to each nucleotide and protein sequence accessible through NCBI search systems.

^d,eTheoretical values of the isoelectric point and molecular weight obtained in the database search using Mascot system.

^fThe species identified with the most significant score for a particular protein. MOWSE, Molecular Weight Search; NCBI, the National Centre for Biotechnology Information; GI, GenInfo Identifier; ACP1, adipocyte acid phosphatase; PKM2, pyruvate kinase PKM.