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. 2018 Feb 8;17(4):5581–5588. doi: 10.3892/mmr.2018.8580

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Copyright: © Lim et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 4. — Flow cytometry analysis of Annexin-FITC/PI stained MCF7-HER18 cells was performed. Following exposure to the indicated treatments for 72 h. (A) B1 quadrant (Annexin⁻, PI⁺) represents necrotic cells, B2 quadrant (Annexin⁺, PI⁺) represents late apoptotic cells, B3 quadrant (Annexin⁻, PI⁻) represents viable cells, and B4 quadrant (Annexin⁺, PI⁻) represents early apoptotic cells. (B) The graph shows the proportions of early and late apoptotic cells following 72 h of treatment as indicated. The data are presented as the mean ± standard deviation. *P<0.05. FITC, fluorescein isothiocyanate; PI, propidium iodide; HER, human epidermal growth factor receptor.