Figure 2.

mRNA and protein expression levels of MMP9, α-SMA, podocalyxin and fibronectin-1 in cultured human podocytes treated with HG. (A) Relative mRNA expression levels of MMP9, α-SMA, podocalyxin and fibronectin-1 wereanalyzed in human podocytes by reverse transcription-quantitative polymerase chain reaction. (B) The relative protein expression levels of MMP9, α-SMA, podocalyxin and fibronectin-1 were detected in human podocytes by western blot analysis. (C) Densito metric analysis of protein expression levels from Part B. *P<0.05 vs. NG; ^#P<0.05 vs. HG. α-SMA, α-smooth muscle actin; HG, high glucose; MMP9, matrix metalloproteinase 9; NG, normal glucose; siRNA, small interfering RNA.