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. 2018 Feb 2;17(4):5642–5651. doi: 10.3892/mmr.2018.8554

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Copyright: © Ling et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 5. — Effects of STZ treatment on expression of MMP9, α-SMA, podocalyxin and fibronectin-1 in DN rats. (A) Expression levels of MMP9, α-SMA, podocalyxin and fibronectin-1 in the podocytes of DN rats were examined dimmunohistochemically. Staining intensity (%), the average positive stained cells percentage to evaluate the slice immunohistochemistry result, measured by Image J software. All the experiments were performed in triplicate. (B) The relative protein expression levels of MMP9, α-SMA, podocalyxin and fibronectin-1 protein in the podocytes of DN rats were examined by western blotting. (C) Demethylation level analysis of MMP9 promoter in vitro. *P<0.05 vs. Control. α-SMA, α-smooth muscle actin; DN, diabetic nephropathy; MMP9, matrix metalloproteinase 9; STZ, streptozotocin.