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Effects of IL-10 on the expression of senescence associated cytokines. Cells were incubated in serum-free medium for 24 h prior to incubation with increasing concentrations of IL-10 for 24 h. Supernatant was collected and levels of (A) IL-6, (B) IL-8 and (C) TNF-α were detected by enzyme linked immunosorbent assays. **P<0.01, ***P<0.001 vs. control group; aP<0.05 vs. 10 ng/ml; bP<0.05 vs. 20 ng/ml. IL, interleukin; TNF-α, tumor necrosis factor-α.
