Figure 1.

Ectopic expression of PBX3 3′UTR promoted HMGB1 expression in HUVECs. (A) Infection efficiency of Lenti-PBX3 3′UTR and Lenti-PBX3-shRNA was measured in HUVECs using RT-qPCR. (B) RT-qPCR and (C) western blotting was applied to examine the mRNA and protein expression levels of PBX3 in HUVECs with PBX3 3′UTR overexpression or knockdown. HMGB1 (D) mRNA and (E) protein expression levels were detected in HUVECs infected with Lenti-PBX3-CDS using RT-qPCR and western blotting. Data are presented as the mean + standard deviation. **P<0.01 vs. Control. RT-qPCR, reverse transcription-quantitative polymerase chain reaction; shRNA, short hairpin RNA; UTR, untranslated region; CDS, coding sequence; PBX3, pre-B cell leukemia transcription factor 3; HMGB1, high mobility group box 1; HUVECs, human umbilical vein endothelial cells.