Table 1.
KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Rabbit monoclonal FOXO3a | Cell Signaling Technology | Cat# 3938, RRID:AB_2106669 |
| Rabbit monoclonal ATG7 | Cell Signaling Technology | Cat# 8558, RRID:AB_10831194 |
| Rabbit monoclonal ATG5 | Cell Signaling Technology | Cat# 9980S, RRID:AB_10829153 |
| Rabbit polyclonal PUMA/BBC3 | Cell Signaling Technology | Cat# 4976, RRID:AB_2064551) |
| Rabbit polyclonal ULK1 | Cell Signaling Technology | Cat# 4773S, RRID:AB_2288252 |
| Rabbit polyclonal PI3 Kinase Class III | Cell Signaling Technology | Cat# 3811S, RRID:AB_2062856 |
| Rabbit polyclonal LC3 | Novus | Cat# NB100-2220, RRID:AB_10003146 |
| Mouse monoclonal beta-Actin | Sigma-Aldrich | Cat# A5441, RRID:AB_476744 |
| Mouse monoclonal p62/SQSTM1 | Novus | Cat# H00008878-M01, RRID:AB_548364 |
| Mouse monoclonal Purified anti-RNA Polymerase II RPB1 antibody Clone H5 | Biolegend | Cat# 920204, RRID:AB_2616695 |
| Bacterial and Virus Strains | ||
| Biological Samples | ||
| Chemicals, Peptides, and Recombinant Proteins | ||
| Bafilomycin A1 | Sigma-Aldrich | B1792; CAS RN: 88899-55-2 |
| Chloroquine | MP Biomedicals | 93919; CAS RN: 50-63-5 |
| Nutlin-3a | Cayman Chemicals | 10004372; CAS RN: 548472-68-0 |
| Protease inhibitor cocktail | Roche | 11836153001 |
| Phosphatase inhibitor | Sigma-Aldrich | P5726 |
| Protein A/G Plus beads | Santa Cruz | SC-2003 |
| Critical Commercial Assays | ||
| Qiagen RNeasy RNA isolation kit | Qiagen | Cat# 74104 |
| QuantiTect Reverse Transcription Kit | Qiagen | Cat# 205311 |
| Deposited Data | ||
| Experimental Models: Cell Lines | ||
| HCT116 | Authenticated | N/A |
| MCF7 | Authenticated | N/A |
| MCF10a | Authenticated | N/A |
| HeLa | N/A | |
| Experimental Models: Organisms/Strains | ||
| Oligonucleotides | ||
| See Table S1 for ChIP primers | This paper | N/A |
| See Table S1 for QPCR primers | This paper | N/A |
| See Table S1 for gRNAs | This paper | N/A |
| Recombinant DNA | ||
| pBABE puro mCherry-GFP-HA-FOXO3a | This paper and Addgene x 2 | #1787 and #1764 |
| Software and Algorithms | ||
| Other | ||
| SYBR Green CFX for QPCR | Applied Biosystems | 4472942 |
| Mirus Transit LT1 transfection reagent | Mirus | MIR2304 |
| CRISPR Max | ThermoFisher | CMAX00001 |
| Cell Event Caspase 3/7 | Invitrogen | C10423 |
| MatTek 35mm dishes for living imaging | MatTek | P35GC-1.5-14-C |
| LysoTracker Blue DND-22 | ThermoFisher | Cat# L7525 |
| Incucyte ZOOM imaging system | Essen BioScience | N/A |