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. 2018 Apr;188(4):853–862. doi: 10.1016/j.ajpath.2017.12.004

Figure 1.

Figure 1

Phosphorylated Y489 β-catenin (p–β-cateninY489) and phosphorylated Y654 β-catenin (p–β-cateninY654) localization in epithelial cells across lung development. A–F: Immunofluorescence (IF) for p–β-cateninY489 (red) and at 14, 18, 21, 31, and 40 weeks, and in adult lung sections. Some p–β-cateninY489 samples are also positive for epithelial cell adhesion molecule (Ep-CAM; green), with frequency of distribution curves (connecting line between means is shown in black) for intensity of p–β-cateninY489 staining. G: Mean intensity of p–β-cateninY489 staining by gestational age. One-way analysis of variance significant for differences between each group. H: IF for p–β-cateninY654 (red) and at 14, 18, 21, 31, and 40 weeks, and in adult lung sections. p–β-cateninY654 is exclusively present in Ep-CAM–positive cells (green). I and J: IF for p–β-cateninY489 (red) and p–β-cateninY654 (red) in neonatal and adult mouse lung sections shows almost no expression. Tiled images compiled as described. Boxed areas in left panels are shown in higher magnification in right panels (A–F and H–J). Data are expressed as means ± SD (G). ∗∗∗∗P < 0.0001. Scale bar = 50 μm (A–F and H–J). Original magnification, ×60 (A–F and H–J).