RNA in situ hybridization for AXIN2 expression in fetal lung, bronchopulmonary dysplasia (BPD) lung, idiopathic pulmonary fibrosis (IPF) lung, and healthy infant and adult controls using RNAscope. A:AXIN2 expression in human fetal, infant, and adult lung sections (red). B:AXIN2 expression in BPD and IPF tissue (red). C: Quantification of AXIN2 expression, normalized per thousand cells counted. There are significant differences between BPD and term infants and IPF and healthy adults. Data are expressed as means ± SD (C). Boxed areas in left panels are shown in higher magnification in right panels (A and B). ∗∗P < 0.01. Scale bar = 20 μm (A and B). Original magnification, ×60 (A and B).