Figure 5.

RCTR2 and RCTR3 limit reflow organ injury and reduce production of leukotriene (LT) B₄ and thromboxane (TxB₂). Hind-limb ischemia-reperfusion (I/R) was induced by applying tourniquets to the hind limbs of 6- to 8-week-old male FVB mice. After 50 minutes, RCTR1, RCTR2, or RCTR3, at 500 ng/mouse i.v., was each directly compared with vehicle (saline containing 0.1% ethanol), followed by removal of tourniquets; reperfusion ensued for 2 hours. Then, animals were sacrificed, and lungs were collected. A: Myeloperoxidase (MPO) levels were assessed by enzyme-linked immunosorbent assay. B: Hematoxylin and eosin histology sections of mouse lungs; arrowheads indicate infiltrated neutrophils. C: Homogenized lungs were extracted and eicosanoids were identified and quantified using lipid mediator metabololipidomics (Materials and Methods). Data are expressed as means ± SEM (A and C). n = 6 to 7 (A–C). ^∗P < 0.05, ^∗∗P < 0.01 versus I/R alone (one-way analysis of variance). Scale bars = 20 μm (B). Original magnification, ×40 (B, main images); ×100 (B, inset).