Figure 6.

RCTR1 accelerates resolution of Escherichia coli infection. FVB mice (males, 6 weeks old) were inoculated with E. coli (10⁵ colony-forming units). RvD1, RCTR1, RCTR2, RCTR3 (100 ng each), or vehicle was given by i.p. injection 12 hours after E. coli inoculation, and peritoneal exudates were collected at 24 and 48 hours. Total leukocytes were enumerated using light microscopy, and polymorphonuclear cell (PMN) numbers were determined by flow cytometry. A: Representative flow cytometry dot plots of phosphatidylethanolamine-Ly6G and fluorescein isothiocyanate–Ly6C. PMNs (Ly6G⁺Ly6C⁻) are denoted in red squares, and monocytes (Ly6C⁺Ly6G⁻) are denoted in black squares. For gating strategy, see Materials and Methods. B: Time courses of PMN numbers. C: Resolution indexes. D: Results are PMN numbers at 24 and 48 hours. Data are expressed as means ± SEM (D). n = 3 to 4 mice per group (A–D). ^∗P < 0.05 versus E. coli + vehicle (unpaired t-test).