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. Author manuscript; available in PMC: 2019 Mar 1.
Published in final edited form as: Immunol Cell Biol. 2017 Dec 29;96(3):273–283. doi: 10.1111/imcb.1036

Figure 1. Generation of intestine specific promoter (Fabpi) driven IL-15 transgenic mice.

Figure 1

The cDNA fragment encoding the open reading frame of murine IL-15 was cloned into the BamHI site present in a 3.5-kb EcoRI fragment containing 1200bp of rat Fabpi promoter linked to 2100bp of the hGH gene. The position of the PCR primers used for the detection of the Fabpi-IL-15 was indicated (A). The intestinal IL-15 mRNA expression of three lines of Fabpi-IL-15 transgenic mice was generated and gene product was quantified by performing real time PCR analysis. (B). The iIL-15 line # 3 showed >10-fold increase of IL-15 protein levels compared to WT mice (C). iIL-15 mice showed induced IFN-γ (D), not TNF-α (E) protein in the jejunum compared to wild type mice. Eosinophil levels in the peripheral blood (F) esophagus (G) and colon (H) have been shown in wild type and iIL-15 transgenic mice. The results are representative of three separate experiments and presented as the mean ± S.D; Experiment “n” = 2, 4 mice/group), NS-not significant.