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. Author manuscript; available in PMC: 2019 Mar 1.
Published in final edited form as: Biochem Pharmacol. 2017 Oct 23;149:186–204. doi: 10.1016/j.bcp.2017.10.011

Figure 4. Effect of (A) NOS inhibition with L-NIO (B) nNOS inhibition with NPLA, (C) nNOS inhibition with 3B7NI or (D) HO inhibition with TINPP on HCT116 cell proliferation.

Figure 4

HCT116 cells were seeded at the density of 6,000 cells per well in xCELLigence plates, treated with various concentrations of the pharmacological inhibitors and proliferation was monitored for 48 h. Left panels show individual xCELLigence experiments run in triplicate wells (mean±SEM); right panels show the summary of 3 experiments conducted on 3 different experimental days for cell proliferation (normalized Cell Index [CI]: analyzed at 48 hours). *P<0.05 shows significant differences in cell proliferation compared to the vehicle control; n=9 determinations (3 experimental days, each performed in triplicate).