Table 3.

Predicted kinases on KEA, transcription factors on ChEA, and hub proteins on PPI Hubs on Enrichr analysis of gene sets that were differentially expressed in podocytes following application of fluid flow shear stress

Predicted Kinases	Predicted Transcription Factors	Predicted Hub Proteins
GSK3β, MAPK1, MAPK3	AR-19668381 (human), AR-20517297 (human), CCND1-20090754 (mouse), CEBPB-20176806 (mouse), CHD1-19587682 (mouse), CLOCK-20551151 (human), CNOT3-19339689 (mouse), CREB1-20920259 (mouse), CREM-20920259 (mouse), CTCF-18555785 (mouse), CTNNB1-20460455 (human), CUX1-19635798 (human), DACH1-20351289 (human), DNAJC2-21179169 (human), E2F1-17053090 (human), E2F1-18555785 (mouse), E2F1-21310950 (human), E2F4-17652178 (human), E2F4-21247883 (human), EGR1-20690147 (human), ELF1-20517297 (human), ELK1-19687146 (human), EOMES-20176728 (mouse), EOMES-21245162 (human), EP300-20729851 (mouse), EP300-21415370 (mouse), ERG-20887958 (mouse), ESR1-17901129 (mouse), ESR1-21235772 (human), ESRRB-18555785 (mouse), EST1-17652178 (human), ETS1-20019798 (human), FLI1-20887958 (mouse), FLI1-21571218 (human), FOXA2-19822575 (human), FOXP2-21765815 (mouse), FOXP3-21729870 (human), GABP-19822575 (human), GATA1-19941827 (mouse), GATA1-21571218 (human), GATA2-19941826 (human), GATA2-20887958 (mouse), GATA2-21571218 (human), GATA2-21666600 (human), GATA3-20176728 (mouse), GATA4-21415370 (mouse), GFI1B-20887958 (mouse), HNF4A-19761587 (human), HNF4A-19822575 (human), HOXB4-20404135 (mouse), JARID1A-20064375 (mouse), JUN-21703547 (human), KDM5B-21448134 (mouse), KLF1-20508144 (mouse), KLF4-18358816 (mouse), KLF4-18555785 (mouse), KLF4-19030024 (mouse), LMO2-20887958 (mouse), MEF2A-21415370 (mouse), MEIS1-20887958 (mouse), MITF-21258399 (human), MTF2-20144788 (mouse), MYB-21317192 (mouse), MYC-18358816 (mouse), MYC-19030024 (mouse), MYC-19079543 (mouse), MYC-19915707 (human), MYC-20876797 (human), MYCN-18555785 (mouse), NACC1-18358816 (mouse), NANOG-16153702 (human), NANOG-16518401 (mouse), NANOG-18347094 (mouse), NANOG-18358816 (mouse), NANOG-18555785 (mouse), NANOG-18692474 (mouse), NANOG-21062744 (human), NFE2L2-20460467 (mouse), NR0B1-18358816 (mouse), NR1I2-20693526 (mouse), NRF2-20460467 (mouse), PADI4-21655091 (human), PAX3-FKHR-20663909 (human), PDX1-19855005 (mouse), PHF8-20622854 (human), POU5F1-16518401 (mouse), POU5F1-18347094 (mouse), POU5F1-18358816 (mouse), POU5F1-18692474 (mouse), PPARD-21283829 (human), PPARG-20176806 (mouse), PPARG-20887899 (mouse), PRDM14-21183938 (mouse), RAD21-21589869 (mouse), RCOR3-21632747 (mouse), REST-18959480 (mouse), REST-19997604 (mouse), REST-21632747 (mouse), RNF2-16625203 (mouse), RUNX1-20887958 (mouse), RUNX1-21571218 (human), SALL4-18804426 (mouse), SALL4-18804426_ESC (mouse), SCL-21571218 (human), SETDB1-19884255 (mouse), SETDB1-19884257 (mouse), SFPI1-20887958 (mouse), SIN3A-21632747 (mouse), SIN3B-21632747 (mouse), SMAD-19615063 (human), SMAD2-18955504 (human), SMAD3-18955504 (human), SMAD3-21741376 (human), SMAD4-21741376 (human), SMARCA4-20176728 (mouse), SOX17-20123909 (mouse), SOX2-16153702 (human), SOX2-18358816 (mouse), SOX2-18692474 (mouse), SOX2-20726797 (human), SOX2-21211035 (human), SPI1-20176806 (mouse), SRF-21415370 (mouse), STAT1-20625510 (human), STAT4-19710469 (mouse), SUZ12-18692474 (mouse), SUZ12-18974828 (mouse), SUZ12-20075857 (mouse), TAL1-20566737 (mouse), TAL1-20887958 (mouse), TBX3-20139965 (mouse), TBX5-21415370 (mouse), TCF3-18347094 (mouse), TCF3-18467660 (mouse), TCF3-18692474 (mouse), TCF4-18268006 (human), TCFAP2C-20176728 (mouse), TCFCP2L1-18555785 (mouse), TET1-21451524 (mouse), TFAP2C-20629094 (human), TFEB-21752829 (human), THAP11-20581084 (mouse), TRIM28-19339689 (mouse), TRP63-18441228 (mouse), WT1-20215353 (mouse), YAP1-20516196 (mouse), YY1-21170310 (mouse), ZFP281-18358816 (mouse), ZFP281-18757296 (mouse), ZFP42-18358816 (mouse), ZFX-18555785 (mouse)	Akt1, ATXN1, CDK1, CDK2, CREBBP, EGFR, EP300, FOS, GSK3B, HDAC1, HSP90AA1, IKBKB, JUN, MAPK1, MAPK3, MAPK8, MYC, NR3C1, PPP2CA, PRKCA, PRKCB, PRKDC, RAC1, RAF1, RELA, RPS6KA3, SFN, SMAD2, SMAD3, SMAD4, STAT1, STAT3, SUMO1, TP53, TRAF6, UBC

Enrichr analysis of genes sets that were differentially expressed (P < 0.01) in podocytes following application of fluid flow shear stress. KEA, Kinase Enrichment Analysis; ChEA, ChIP-x Enrichment Analysis; PPI Hubs, Protein-Protein Interaction Hubs. Transcription factors: AR, androgen receptor; CCND1, cyclin-D1; CEBPB, CCAAT/enhancer-binding protein-β; CHD1, chromodomain-helicase-DNA-binding protein-1; CLOCK, basic helix-loop-helix-PAS transcription factor; CNOT3, C-C chemokine receptor type 4 (CCR4)-NOT transcription complex subunit 3; CREB1, cAMP-response element-binding protein-1; CREM, cAMP-response element modulator; CTCF, CCCTC-binding factor; CTNNB1, β-catenin; CUX1, cut-like homeobox 1; DACH1, Dachshund homolog 1; DNAJC2, dnaJ homolog subfamily C member 2; E2F, E2 factor; EGR1, early growth response protein-1; ELF1, E74-like factor 1; ELK1, erythroblast transformation-specific (ETS) domain-containing protein-1; EOMES, eomesodermin; EP300, E1A-associated protein p300; ERG, ETS-related gene; ESR1, estrogen receptor 1; ESRRB, estrogen-related receptor-β; EST1, telomerase-activating protein-1; ETS1, E26 transformation-specific sequence-1; FLI1, friend leukemia integration 1; FOX, forkhead box protein; GABP, GA-binding protein; GATA1-4, GATA-binding factor 1–4; GFI1B, zinc finger protein Gfi-1b; HNF4A, hepatocyte nuclear factor 4α; HOXB4, homeobox protein Hox-B4; JARID1A, lysine-specific demethylase 5A; KDM5B, lysine-specific demethylase 5B; KLF, Krueppel-like factor; LMO2, LIM domain only 2; MEF2A, myocyte-specific enhancer factor 2A; MEIS1, homeobox protein Meis1; MITF, microphthalmia-associated transcription factor; MTF2, metal-response element-binding transcription factor 2; MYB, Myb proto-oncogene protein; MYC, Myc proto-oncogene protein; MYCN, N-myc proto-oncogene protein; NACC1, nucleus accumbens-associated protein-1; NANOG, homeobox protein Nanog; NFE2L2, nuclear factor (erythroid-derived 2)-like 2; NR0B1, nuclear receptor subfamily 0 group B member 1; NR1I2, nuclear receptor subfamily 1 group I member 2; NRF2, nuclear factor E2-related factor 2; PADI4, protein-arginine deiminase type 4; PAX3-FKHR, paired box protein Pax-3-FKHR protein; PDX1, pancreas/duodenum homeobox protein-1; PHF8, plant homeodomain finger protein-8; POU5F1, POU domain, class 5 transcription factor 1; PPARD and PPARG, peroxisome proliferator-activated receptor-δ and -γ, respectively; PRDM14, PR domain zinc finger protein-14; RAD21, double-strand-break repair protein rad21 homolog; RCOR3, repressor element-1 silencing transcription factor (REST) corepressor 3; RNF2, E3 ubiquitin-protein ligase RING2; RUNX1, runt-related transcription factor 1; SALL4, Sal-like protein-4; SCL, stem cell leukemia transcription factor; SETDB1, histone-lysine N-methyltransferase Setdb1; SFPI1, transcription factor PU.1; SIN, paired amphipathic helix protein; SMAD, mothers against decapentaplegic homolog; SMARCA4, transcription activator BRG1; SOX, SRY-related high-mobility group (HMG) box transcription factor; SPI1, transcription factor PU.1; SRF, serum response factor; SUZ12, polycomb protein Suz12; TAL1, T cell acute lymphocytic leukemia protein-1; TBX, T-box transcription factor; TCF3 and TCF4, transcription factor 3 and 4, respectively; TCFAP2C, transcription factor AP-2γ; TCFCP2L1, transcription factor CP2-like protein-1; TET1, methylcytosine dioxygenase Tet1; TFAP2C, transcription factor AP-2γ; TFEB, transcription factor EB; THAP11, THAP domain-containing protein-11; TRIM28, transcription intermediary factor 1β; TRP63, tumor protein 63; WT1, Wilms tumor protein homolog; YAP1, Yes-associated protein-1; YY1, transcriptional repressor protein Yy1; ZFP281, zinc finger protein; ZFX, zinc finger X-chromosomal protein. Hub proteins: ATXN1, ataxin-1; CDK, cyclin-dependent kinase; CREBBP, CREB-binding protein; EGFR, EGF receptor; HDAC1, histone deacetylase 1; HSP90AA1, heat shock protein Hsp 90α; IKBKB, inhibitor of NF-κB kinase subunit-β; NR3C1, glucocorticoid receptor; PPP2CA, serine/threonine-protein phosphatase 2A catalytic subunit α-isoform; PRKCA and PRKCB, protein kinase C α- and β-type, respectively; PRKDC, DNA-dependent protein kinase catalytic subunit; RAC1, Ras-related C3 botulinum toxin substrate 1; RAF1, RAF proto-oncogene serine/threonine-protein kinase; RELA, transcription factor p65; RPS6KA3, ribosomal protein S6 kinase-α3; SFN, 14-3-3 protein-σ; SUMO1, small ubiquitin-related modifier 1; TP53, cellular tumor antigen p53; TRAF6, TNF receptor-associated factor 6; UBC, polyubiquitin-C.