Fig. 2.

Fenofibrate treatment augments PPARα expression. 50-day-old Pkd1^RC/RC mice were randomized to receive either standard chow diet (Ctl) or a diet supplemented with fenofibrate (Feno) for 5 mo. At 200 days of age, these mice were euthanized for analysis. A–C: Western blot analysis of total kidney lysate (A) and subsequent quantification (B and C) demonstrated increased expression of PPARα and PMP-70, a marker of peroxisomes, in fenofibrate-treated Pkd1^RC/RC mice (n = 5) compared to control-treated Pkd1^RC/RC mice (n = 5). D: immunofluorescence staining revealed that fenofibrate-treated Pkd1^RC/RC mice showed increased expression of PPARα (red) and PMP-70(red) in kidney cyst epithelial cells compared to control-treated Pkd1^RC/RC mice. Error bars indicate means ± SE.