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. 2017 Oct 26;314(1):G131–G141. doi: 10.1152/ajpgi.00103.2017

Fig. 6.

Fig. 6.

Diet-, binge-, and time-dependent effects on markers of liver pathology in Nanji diet-fed mice. Mice were fed a Nanji diet for 4 wk, and during the 5th wk (i.e., days 43–45) mice were administered a once/daily binge of alcohol (6 g/kg) or the vehicle control for alcohol (i.e., PBS) at a specific time indicated by the zeitgeber time (ZT), and mice were euthanized 4 h after the 3rd and final once/daily binge. Liver pathology was assessed using a combination of histological and serum analyses. A: liver steatosis exhibited a significant effect of binge (P = 0.01) and time (P < 0.00) (nonparametric Kruskal-Wallis). Post hoc Tukey’s test showed significant time-of-day effects. B: liver inflammation exhibited a significant effect of time (P < 0.00) (nonparametric Kruskal-Wallis). Post hoc analysis revealed significant time-of-day effects. C: liver myeloperoxidase (MPO) exhibited a significant effect of binge (P < 0.00). Post hoc analysis revealed no significant effects. D: serum alanine aminotransferase (ALT) exhibited a significant effect of time (P = 0.01) as well as a binge × time interaction (P = 0.02). Post hoc Tukey’s test revealed only a single time-of-day difference. E: serum aspartate aminotransferase (AST) exhibited a significant effect of binge (P = 0.00) and time (P = 0.01). Post hoc Tukey’s test revealed no significant differences. Two-way ANOVA followed by post hoc Tukey’s test (*P < 0.05; **P < 0.01; ***P < 0.001) were used for analysis unless noted.