Fig. 2.

UT-A1 immunoprecipitation. A: a representative UT-A1 immunoblot of immunoprecipitation (IP) experiment. IMCD suspensions were incubated with vehicle or dDAVP (1 nM for 15 min), followed by in-cell cross-linking using BSOCOES, RIPA-solubilization, and immunoprecipitation using UT-A1 antibody or preimmune IgG-laden Dynabeads as control. The IP input was the RIPA-solubilized fraction. Equal volumes of IP products were loaded for immunoblotting using the UT-A1 antibody. The same membrane was reprobed, without stripping, with the UT-A3 antibody (B), and then with the aquaporin 2 (AQP2) antibody (C).