Fig. 6.

AQP2 immunoprecipitation. A representative AQP2 immunoblot of immunoprecipitation experiment is shown. IMCD suspensions were incubated with vehicle or dDAVP (1 nM for 15 min), followed by in-cell cross-linking using BSOCOES, RIPA solubilization, and immunoprecipitation using AQP2 antibody or preimmune IgG-laden Dynabeads as control. The IP input was the RIPA-solubilized fraction. Equal volumes of IP products were loaded for immunoblotting using the AQP2 antibody (lanes 3–6). Unbound, the supernatant collected after overnight incubation of IP input with AQP2 antibody-laden Dynabeads.