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. 2017 Oct 18;314(1):C62–C72. doi: 10.1152/ajpcell.00162.2017

Fig. 3.

Fig. 3.

Effect of transcription factor EB (TFEB) overexpression in myotubes. Real-time PCR analysis of mRNA from cells overexpressing adenoviral TFEB. A: Western blot indicating successful overexpression of TFEB. Effect of TFEB overexpression on autophagy mRNA markers (B) and mitochondrial biogenesis mRNA level (C). Transcript levels are normalized to both β-actin and GAPDH (n = 3–6; *P < 0.05 mock green fluorescent protein (GFP) vs. TFEB overexpression; Student’s t-test on GFP vs. TFEB overexpression on raw data). NUC, nuclear; CYT, cytosolic; LC3, microtubule-associated proteins-1A/1B light chain 3A; LAMP2, lysosomal associated membrane protein-2; CATH D, cathepsin D; PGC-1α, peroxisome proliferator-activated receptor-γ coactivator-1α; COX, cytochrome-c oxidase; TFAM, mitochondrial transcription factor A.