Fig. 7.

Transcription factor EB (TFEB) transcription with exercise in vivo. TFEB transcription was analyzed in wild-type (WT) and peroxisome proliferator-activated receptor-γ coactivator-1α (PGC-1α) knockout (KO) mice following an acute bout of exercise. A: TFEB transcription measured as luciferase activity (n = 7–9; ¶P < 0.05, interaction effect indicating increase with exercise only in WT animals; 2-way ANOVA). R.L.U, relative light units. B: mRNA levels of TFEB in WT and PGC-1α KO mice before and after an acute bout of exercise. Gapdh and Actb were used as housekeeping genes (n = 6–7). A.U, arbitrary units. C: PGC-1α translocation to the nucleus following exercise was measured in WT mice. PGC-1α nuclear content as a percentage of total [cytosolic (C) and nuclear (N)] signals. Histone H2B and α-tubulin were used as nuclear and cytosolic purity controls (n = 7–9, *P < 0.05 WT control vs. WT exercise; Student’s t-test). CON, control; EX, exercise.