Skip to main content
. 2017 Oct 4;314(1):C53–C61. doi: 10.1152/ajpcell.00175.2017

Fig. 7.

Fig. 7.

Effects of sclerostin on gene expression. Bovine trabecular bone was prepared as described in materials and methods and cultured ex vivo (without loading) with different doses of rhSCL (0–100 ng/ml). Total RNA was extracted at 72 h and real-time RT-PCR performed for CA2 (A), CTSK (B), TRAP (C), RANKL (D), MEPE (E), and PHEX mRNA (F). Data are mean expressions normalized to that of the housekeeping gene BACT mRNA ± SD for triplicate RT-PCR reactions from quadruplicate bone samples/group. Difference in expression from the untreated control is indicated (*P < 0.05).