Effects of sclerostin on gene expression. Bovine trabecular bone was prepared as described in materials and methods and cultured ex vivo (without loading) with different doses of rhSCL (0–100 ng/ml). Total RNA was extracted at 72 h and real-time RT-PCR performed for CA2 (A), CTSK (B), TRAP (C), RANKL (D), MEPE (E), and PHEX mRNA (F). Data are mean expressions normalized to that of the housekeeping gene BACT mRNA ± SD for triplicate RT-PCR reactions from quadruplicate bone samples/group. Difference in expression from the untreated control is indicated (*P < 0.05).