Table 1.

Sequences of oligonucleotide primers for real-time RT-PCR designed on the basis of published bovine gene sequences and predicted PCR product sizes

Target Gene	Sense	Primer Sequence (5′→3′)	Expected Product Size, bp
BACT	S	ACCGTGAGAAGATGACCCAGA	127
	AS	TCACCGGAGTCCATCACG
SOST	S	CCCTTTGAGACCAAAGACGC	244
	AS	CAGGACACAACAGCTGCACC
CA2	S	GGAAGAAATATGCTGCCGAGC	194
	AS	GAAGTCGGTGCTCTTACCCTTTG
TRAP	S	GCAGCCAAGGAGGACTACGTG	176
	AS	CCATTCTCATCCTGAAGGTACTGC
CTSK	S	CCTATCCATATGTTGGACAGGATG	169
	AS	AAGGAGGTCAGGCTTGCATC
RANKL	S	CTACTTCCGAGCGCAGATGG	90
	AS	TCCGTGTTTTCATGGAGCTTG
MEPE	S	TGACCCTGGCAGCACCAAC	219
	AS	GCTCTTGACTTCTCTTGCCAGAATG
PHEX	S	GGGTGTTCGATGGGCCTTTA	217
	AS	ATACTTGCCGGTTTGCAGGA

S, sense; AS, antisense.