Table 2.
Analytic detection of EO in mammals
| Senior/Key Author(s) (Location) | Year | Source | Material | Analytical Method | Reference |
|---|---|---|---|---|---|
| A. Positive detection of EO in mammals (published spectra/MS and NMR data) | |||||
| 1. Hamlyn/Blaustein/DuCharme (Baltimore, MD/Kalamazoo, MI) | 1991 | Human | Plasma | MS | (118) |
| 2. Mathews/DuCharme/Hamlyn (Kalamazoo/Baltimore) | 1991 | Human | Plasma | MS | (199) |
| 3. Inagami/Tamura (Nashville, TN) | 1994 | Rat | Urine | MS, NMR | (276) |
| 4. Doris (Lubbock, TX) | 1994 | Bovine | Adrenal | ——* | (59) |
| 5. Defaye/Perrin (Grenoble, France) | 1997 | Bovine | Adrenal | MS | (232) |
| 6. Schoner/Schneider (Giessen, Germany) | 1998 | Bovine | Adrenal | MS, NMR | (256) |
| 7. Nakanishi/Kawamura/Haupert (New York, NY/Boston, MA) | 1999 | Bovine | Hypothalamus | NMR | (149) |
| 8. Takahashi/Komiyama (Tokyo, Japan) | 2000 | Human | Plasma | MS | (155) |
| 9. Manunta/Hamlyn/Pitzalis (Milan, Italy/Baltimore) | 2005 | Human | Plasma | MS | (234) |
| 10. Hamlyn/Manunta (Baltimore/Milan) | 2006 | Human | Plasma | MS | (191) |
| 11. Hamlyn/Jacobs (Baltimore/Milan) | 2012 | Rat | Plasma | MS | (138) |
| 12. Hamlyn/Leenen/Blaustein (Baltimore/Ottawa, Canada) | 2014 | Rat | Plasma | MS | (121) |
| 13. Hamlyn/Blaustein (Baltimore) | 2016 | Rat/Mouse | Plasma | MS | (117) |
| B. Identification of EO isomers (published spectra) | |||||
| 1. Hamlyn/Jacobs (Baltimore) | 2012 | Rat | Plasma | MS | (138) |
| 2. Hamlyn/Leenen (Baltimore/Ottawa) | 2014 | Rat | Plasma | MS | (121) |
| C. Did not identify EO in human samples | |||||
| 1. Doris (Lubbock, TX) | 1994 | Human | Plasma | RIA† | (59) |
| 2. Nicholls/Lewis (Christchurch, New Zealand) | 1994 | Human | Plasma | ELISA† | (173) |
| 3. Hilton (London, UK) | 1996 | Human | Placenta | MS | (126) |
| 4. Vogeser/Baecher (Munich) | 2014 | Human | Plasma | MS‡ | (12, 289) |
*
Identified by elution pattern.
†
Not a definitive analytical method.
‡
The MS trace signal is broken at ∼5.0 min in the original publication (Fig. 2, upper spectrum) (12), but an ion current at that position is shown in spectra from two patients later published in a letter to the editor (289) (and see Ref. 117 online supplement Fig. S7). That ion current had the same mass/charge ratio as ouabain but eluted 0.3 min ahead of ouabain.