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PCR primers
| Gene | Forward Primer (5′→3′) | Reverse Primer (5′→3′) | Ref. |
|---|---|---|---|
| Ppib | GGAGATGGCACAGGAGGAA | GCCCGTAGTGCTTCAGCTT | 19 |
| Ucp1 | ACTGCCACACCTCCAGTCATT | CTTTGCCTCACTCAGGATTGG | 19 |
| Ppargc1a | CCCTGCCATTGTTAAGACC | TGCTGCTGTTCCTGTTTTC | 3 |
| Prdm16 | CAGCACGGTGAAGCCATTC | GCGTGCATCCGCTTGTG | 3 |
| Cidea | TGCTCTTCTGTATCGCCCAGT | GCCGTGTTAAGGAATCTGCTG | 3 |
