Fig. 9.

Endothelin-1 (ET-1) promotes monocyte-endothelial cell adhesion in part via TRAF3 interacting protein 2 (TRAF3IP2). Human aortic endothelial cells (HAECs) at 50% confluency were infected with lentiviral particles expressing TRAF3IP2 or control eGFP shRNA [multiplicity of infection (MOI) 0.5 for 48 h], treated with ET-1 (10 nM) for 12 h, and then incubated with calcein AM-loaded THP-1 monocytic cells for 1 h. Monocyte-endothelial cell adhesion was quantified by measuring fluorescence at excitation and emission wavelengths of 485 and 535 nm, and relative fluorescence intensity was quantified. Wells containing HAECs without THP-1 cells served as blanks. *P ≤ 0.01 vs. control (i.e., open bar); †P ≤ 0.01 vs. ET-1 (n = 6).