Fig. 5.
The Notch1 intracellular domain (NICD) affects fast and slow components of the transient outward current (Ito). A: whole cell voltage-gated K+ currents recorded in voltage clamp in a green fluorescent protein (GFP)-negative myocyte isolated from the intraventricular septum of a wild-type (WT) mouse (MCM-WT) and a GFP-positive cell from the intraventricular septum of a transgenic mouse with NICD expression (MCM-NICD-GFP). The voltage-clamp protocol consisting of a 4.5 s-depolarizing step is shown at the bottom. B: fitted curves of current traces shown in A with triexponential function (black curves) and relative monoexponential fitted components (shades of gray curves). C: quantitative data for the amplitude of Kv current components in GFP-negative myocytes from the ventricular septum of MCM-WT mice (WT, n = 18; from 3 animals) and GFP-positive myocytes from the ventricular septum of MCM-NICD-GFP mice (NICD, n = 17; from 5 animals) are shown as means ± SE. *P < 0.01 vs. WT.