Fig. 1.

Deletion of Cif (cystic fibrosis transmembrane conductance regulator inhibitory factor) slows PA14 inhibition of mucociliary transport (MCT). A: timeline of the experiment. B: view of bead locations in one positional axis (horizontal axis) over time (vertical axis) from the epithelium of donor 3. C: diagram of the velocity calculation for each bead shown as a point in D. D: quantification of individual bead velocities from each condition between time point 12 min 0.0 s and time point 12 min 10.0 s, stratified by donor ID. P values indicate significance derived from log₂ transformation and modeling as described in the text and in H. E: maximum intensity projection of the movie from the control condition between the 10- and 15-min marks from donor 3. F: projection from the same donor and capture as E, where PA14 was added to the apical side of the culture dish. G: projection from the same donor and capture as E, where PA14 Δcif was added to the apical side of the culture dish. H: results of a random-intercept mixed-effect linear model used to assess the magnitude and statistical significance of differences in log₂-transformed bead velocities associated with treatment with vehicle control, PA14, or PA14 Δcif.