Fig. 6.

Sucralose attenuates LPS-induced elevated heat shock protein 27 (HSP27) and p110α phosphatidylinositol 3-kinase (p110αPI3K) and activation of myosin light chain-2 (MLC2), Src, and p21-activated kinase (PAK). Rat LMVECs were treated in the presence or absence of LPS (1 µg/ml) and sucralose (0.1 mM) for 24 h. Phosphorylation of MLC-2 (A), Src (B), and PAK (C) was assessed in whole cell lysates by immunoblot analysis with an antibody specific to each phosphorylated protein. Blots were stripped and reprobed for total protein expression and actin as a loading control. Total protein expression of HSP27 (D) and p110αPI3K (E) was also assessed in whole cell lysates, followed strip and reprobe of blots for actin as a loading control. Representative blots are shown. Nonessential lanes from the HSP27 representative blot (D) have been removed; n = 6. Data are expressed as means ± SD. *P < 0.05 vs. vehicle for LPS.