Fig. 1.

Chronic hypoxia (CH) increases acid-sensing ion channel 1 (ASIC1) plasma membrane localization in pulmonary arterial smooth muscle cells (PASMC). A and B: representative Western blot (WB) for total protein homogenate and corresponding Coomassie-stained blot and summary data detecting total ASIC1 expression (normalized to the entire lane on the Coomassie-stained blot) in PASMC from control and CH rats. C and D: representative Western blots for biotinylated (plasma membrane) and cytosolic protein and summary data showing the ratio of biotinylated to cytosolic ASIC1 expression in PASMC from control and CH rats. MW, molecular mass (kDa). ○, Individual data points; bars, means ± SE of number of animals shown in parentheses. *P ≤ 0.05 vs. control (by unpaired t-test).