Fig. 2.

RhoA stimulates ASIC1 plasma membrane localization in PASMC from control and CH rats. Representative Western blots for biotinylated (plasma membrane) and cytosolic (A and C) protein and summary data (B and D) showing the ratio of biotinylated to cytosolic ASIC1 expression in PASMC from control (Con, B) and CH (D) rats following treatment with Rho inhibitor I (1 µg/ml), Rho activator II (1 µg/ml), and the Rho kinase inhibitor Y-27632 (10 µM). Con vehicle and CH vehicle (D) are the same samples shown in Fig. 1D. ○, Individual data points; bars, means ± SE of number of animals shown in parentheses. *P < 0.05 vs. control; #P < 0.05 vs. respective vehicle [by 1-way ANOVA and Student-Newman-Keuls test (for individual group comparisons)].