Fig. 7.

Inhibition of ASIC1a does not influence CH-induced activation of RhoA in isolated pulmonary arteries. A and B: representative images of GTP-bound activated RhoA (~22 kDa) and total RhoA (~22 kDa) in intrapulmonary arterial homogenates from control (Con) and CH rats preincubated in the presence of vehicle or PcTX1. IP, immunoprecipitation. C: summary data showing expression of GTP bound to total RhoA. ○, Individual data points; bars, means ± SE of number of animals shown in parentheses. *P ≤ 0.05 vs. corresponding Con [by 2-way ANOVA and Student-Newman-Keuls test (for individual group comparisons)].