Fig. 1.

H⁺-K⁺-ATPase type 2 (HKA2) antibody specificity was confirmed by peptide absorption. The specific band at 100 kDa was visible only in colon (COL) in both total tissue homogenate and membrane fraction and was greatly reduced when the HKA2 antibody was preincubated with immunizing peptide. Only nonspecific background bands, unaffected by immunizing peptide, were visible in the whole renal cortex (CTX), membrane fraction of the renal CTX, and outer medulla (OM) of virgin (V) rats. Samples were from a virgin rat and were all loaded at 50 µg of protein.