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. 2017 Oct 18;314(2):F251–F259. doi: 10.1152/ajprenal.00288.2017

Fig. 5.

Fig. 5.

Immunolocalization of ROMK. Apical ROMK in the aldosterone-sensitive distal nephron diminishes in pregnancy. Representative images of ROMK in the late distal convoluted tubule (DCT2; A) and connecting tubule (CNT; B) of virgin and late pregnant rats. DCT2 was identified by the presence of calbindin and absence of aquaporin 2 (AQP2); CNT was identified by the presence of calbindin and AQP2. Bar = 5 μM. C: representative line scan measurements of ROMK pixel intensity (shown for the cells on the left, labeled a and b. Apical membrane delimited ROMK pixel intensity is taken as peak pixel intensity within 1 μm of the lumen minus the local cytoplasmic intensity (within 2–3 μM from the lumen). D: summary data of apical membrane delimited ROMK pixel intensity in DCT2 and CNT of virgin (n = 3) and late pregnant rats (n = 4). Bar graphs are means ± SE of n = 3–4 rats; individual data points are average intensity of >30 cells from each animal. *P < 0.05 vs virgin by unpaired t-test.