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. 2017 Oct 11;314(2):F293–F305. doi: 10.1152/ajprenal.00364.2017

Fig. 6.

Fig. 6.

P2X7 receptor activation selectively increases mouse kidney PAD4 mRNA in vivo. PAD4 wild-type mice were treated with vehicle or with 5 mg/kg BzATP ip 15 min before sham surgery or 20 min renal ischemia and 24 h reperfusion. A: PAD4 and PAD2 mRNA expressions in kidney cortexes were measured with quantitative RT-PCR. BzATP (a selective P2X7 receptor agonist) induced PAD4 but not PAD2 mRNA expression in mouse kidney (BzATP Sham). Renal IR also selectively induced PAD4 mRNA expression in mouse kidney (Vehicle 20 min RIR) and this PAD4 induction was significantly amplified with BzATP treatment (BzATP 20 min RIR). No change in PAD2 mRNA expression was observed in mouse kidney 24 h post-RIR (N = 5–7 experiments). B: PAD4 activity in mouse kidney cortexes. BzATP as well as 20 min RIR increased kidney PAD4 activity in sham-operated mice. *P < 0.05 vs. Vehicle Sham, #P < 0.05 vs. Vehicle 20 min RIR. Error bars represent 1 SE.