Figure 2.

Protein expression of glycolytic regulators in hACs on different treatments. Representative Western blots and quantifications of glycolytic proteins in cell cultures treated with vehicle (control), TGF-β1 (5 ng/ml) or BMP2 (100 ng/ml) for 72 h. TGF-β1–treated cells have increased protein expression of Glut1, HKI and HKII compared with the control. BMP2-treated cells have slightly increased protein expression of Glut1 and HKI, with HKII unchanged, compared with the control. Data are means ± SD of four independent experiments (*p < 0.05 by two-tailed t test).

hACs = human articular chondrocytes; HKI = hexokinase I; HKII = hexokinase II; SD = standard deviation.