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Binding kinetics of SRRP53608-BR variants against PGA as analyzed by bio-layer interferometry
| SRRP53608-BR variants | Kd (M) | ka, M−1⋅s−1 | ka error | kd, s−1 | kd error | Full χ2 | Full R2 |
| Wild-type | 2.37E-07 | 3.03E+02 | 1.64E+00 | 7.17E-05 | 4.92E-06 | 0.231987 | 0.997077 |
| ΔF411–T422 | 2.84E-06 | 3.14E+02 | 2.71E+00 | 8.91E-04 | 9.29E-06 | 0.314129 | 0.993248 |
| R512A | 4.66E-06 | 2.46E+02 | 2.59E+00 | 1.15E-03 | 1.03E-05 | 0.094123 | 0.992496 |
| K377A | 5.24E-06 | 2.58E+02 | 3.92E+00 | 1.35E-03 | 1.59E-05 | 0.114659 | 0.985439 |
