Fig. 2.

Inactivation of Bpnt1 specifically in the small intestine results in iron-deficiency anemia. (A) Representative photograph of Wright-stained whole blood smear from Bpnt1^−/fl and Bpnt1^−/int showing microcytic hypochromic RBCs. (Scale bar: 25 μm.) (B and C) Hematological parameters of Bpnt1^−/fl and Bpnt1^−/int 8-wk-old mice demonstrating anemia (n = 6–8 per group). (D) Quantification of tissue iron stores in livers and spleens of 8-wk-old male Bpnt1^−/fl and Bpnt1^−/int mice. (E) qRT-PCR of Hamp1 mRNA relative to beta actin from total liver RNA in Bpnt1^−/fl and Bpnt1^−/int mice (n = 4). *P < 0.05.