Fig. 3.

Bpnt1^−/int enterocytes have defects in apical iron transport. (A) Hb levels of mice on control or iron-deficient diets. Mice were fed a low iron (2 ppm) or control (200 ppm) diet for 5 wk after weaning (n = 3–5 per group). (B) qRT-PCR of Dmt1+IRE mRNA relative to actin in the duodena of control or iron starved mice (n = 3–5 per group). (C) Western blot analysis of Dmt1, Bpnt1, and actin loading control from isolated enterocytes of the same mice as in B. *P < 0.05.