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View full-text article in PMC

. 2018 Feb 13;115(12):3090–3095. doi: 10.1073/pnas.1715530115

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Fig. 4. — Characterization of select evolved recoded clones for applications featuring nsAAs. (A) Expression of a control GFP reporter containing no UAG codons in various strains in the presence and absence of the nsAA p-acetyl-phenylalanine (pAcF). (B) nsAA incorporation assay based on expression of a GFP reporter containing two UAGs in various strains in the presence and absence of pAcF. Evolved recoded clone G5-1 was selected as C321.∆A.M9adapted for deposit in the Addgene repository. (C) Doubling time measurements for C321.∆A.M9adapted and related strains in glucose minimal media.