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. 2018 Mar 5;115(12):2964–2969. doi: 10.1073/pnas.1716920115

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Fig. 5. — Glucaric acid production from strains with layered dynamic regulation at 72 h. (A) Glucaric acid titer as a function of Pfk-1 switching time (QS module variants) and Miox expression time (MI biosensor circuit variants) at the 250-mL shake flask scale. Error bars represent ±1 SD from the mean of three replicate flasks. (B) Glucaric acid production at the 3-L bioreactor scale for strains L19GA + P2 and L19GA + no IpsA. Error bars represent ±1 SD from the mean of two replicates. No IpsA, constitutive (unregulated) Miox expression; IB1379GA, wild-type pfkA expression (no knockdown of pfkA).