Fig. 6.
SAXS profiles of the chimeras. (A) SAXS profiles of TmaACT-GspDAH7PS were measured in the absence (light blue circles) and presence (pink circles) of Tyr. Theoretical scatterings were calculated from Tyr-free (blue line) and Tyr-bound (red line) TmaDAH7PS crystal structures. (B) Kratky plot of TmaACT-GspDAH7PS SAXS profiles in the absence (blue circles) and presence (red circles) of Tyr. (C) SAXS profiles of GspCM-TmaDAH7PS were measured in the absence (light blue circles) and presence (pink circles) of prephenate. Theoretical scatterings were calculated from prephenate-free model (blue line) and prephenate-bound (red line) crystal structure of GspDAH7PS. (D) Kratky plot of GspCM-TmaDAH7PS SAXS profiles in the absence (blue circles) and presence (red circles) of prephenate. I, scattering intensity; S, magnitude of scattering vector (where S = 4πsinθ/λ, 2θ is the scattering angle and λ is the wavelength of the radiation).